Nuclear fragmentation is a consequence of programmed cell death by activation of caspase cascade resulting in disruption of nuclear lamina integrity 19 in this study nuclear lamina damage was observed after lamin b1 immunostaining fig. Caspase 9 is an enzyme that in humans is encoded by the casp9 gene it is an initiator caspase critical to the apoptotic pathway found in many tissues. Inhibition of caspase activity revealed that the bulk of the lamin b both b1 and b2 was mislocalized to the cytoplasm excluding the trivial possibility that failure to incorporate lamin b into the nuclear lamina was due to lamin degradation.
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